Characterization of CD34+ cells from canine umbilical cord blood, bone marrow leukocytes, and peripheral blood by flow cytometric analysis |
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| Authors: | Tsumagari Shigehisa Otani Isao Tanemura Koichi Namba Shinichi Ohtaki Tadatoshi Kamata Hiroshi Kanayama Kiichi |
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| Institution: | Laboratory of Therigenology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fukjsawa, Kanagawa 252-8510, Japan. tsumagar@brs.nihon-n.ac.jp |
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| Abstract: | Characterization of CD34+ cells in canine bone marrow, umbilical cord blood, and peripheral blood was performed by flow cytometric analysis. The ratio of CD34+CD45hi cells, which are absent in human blood, was high in the CD34+ cell fraction, but 98% of these was suggested B-cells. The remaining CD34+CD45lo cells may comprise canine hematopoietic progenitor cells, and these cells accounted for 0.23 +/- 0.07% of the fraction in cord blood, 0.30 +/- 0.07% in bone marrow, and 0.02 +/- 0.01% in peripheral blood. |
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