云南特有茶树种质资源遗传多样性的RAPD研究

运用RAPD技术对云南主产茶区具有代表性的野生型古茶树、过渡型古茶树、栽培型品种、野生种、地方品种及其近缘植物———金花茶等48份材料进行遗传多样性分析,从核基因组DNA的角度深入探讨了云南特有茶树种质资源的分类及其亲缘关系。用已筛选出的12个引物对48份供试材料进行RAPD扩增反应后,共检测到112条扩增片段,所有片段均为多态,其多态性程度高达100%.材料间的遗传距离为0 116～0 527,平均为0 202.对48份供试材料间的亲缘关系进行UPGMA聚类分析,研究结果表明:当以欧氏距离为6 0来划分时,可分为5个组,其中3个复合组,2个独立组,基本上与传统分类水平相吻合。

Study on the Genetic Diversity of Peculiar Tea Germplasm Resource in Yunnan by RAPD

Random Amplified Polymorphic DNA (RAPD) technique was used to analysis the genetic diversity of 48 materials,Which including wild type old tea tree,intermediate type old tea tree,cultivated type species,wild species,local variety and the kidney plant:Camellia fascicularis. The classification and blood relationship among 48 germplasm were explored from DNA level. No single-morphic band has been found among the total 112 DNA bands. The genetic diversity degree was 100%. The figure showed that the tea trees in Yunnan processing much high genetic diversity on DNA molecular level. The genetic distance was between 0.116 to 0.527 and the average was 0.202. All the RAPD amplified bands were clustered by Unweighted Pair Group with Mathematic Average (UPGMA) based on Euclidean distances. The dendrogram of UPGMA showed that the 48 materials could be classified into 5 groups including 3 complex groups and 2 simple groups, which was basically identical with morphological classification.