| PIWIL1基因启动子调控的特异表达Cre重组酶的转基因猪的构建 |
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| 引用本文： | 段新平,高飞,武蓉,宋奇,韩杨,姚潮刚,纪元.PIWIL1基因启动子调控的特异表达Cre重组酶的转基因猪的构建[J].安徽农业科学,2012,40(9):5231-5233. |
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| 作者姓名： | 段新平 高飞 武蓉 宋奇 韩杨 姚潮刚 纪元 |
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| 作者单位： | 吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062;吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062;吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062;吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062;吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062;吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062;吉林大学畜牧兽医学院,动物胚胎工程吉林省重点实验室,吉林长春130062 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助(2011CB944203) |
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| 摘 要： | 目的]建立PIWIL1基因启动子调控的特异性表达Cre重组酶的转基因猪,为研究piRNA及其相关基因的功能和机制奠定基础。方法]通过PCR扩增,从猪的全基因组上扩增出PIWIL1基因的启动子。切除猪源Cre重组酶的表达载体pET28a-MHC-Cre-BGHpo1yA-FRT2neor的启动子MHC,将其与PIWIL1基因启动子连接,构建成特异性表达Cre重组酶的pPIWIL1-Cre载体。将该载体转染小型猪胎儿成纤维细胞,通过G418筛选,获得阳性克隆进行核移植。结果]获得2头PIWIL1基因启动子调控的特异表达Cre酶的转基因猪。结论]成功构建了PIWIL1基因启动子调控的特异表达Cre重组酶的转基因猪,为研究piRNA及其相关基因的功能和机制提供了工具猪。
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| 关 键 词： | piRNA PIWIL1 Cre酶 转基因猪 |
Construction of Transgenic Swine Specifically Expressing Cre Recombinase Controlled by PIWIL1 Promoter |
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| Institution: | DUAN Xin-ping et al(Jilin Provincial Key Laboratory of Animal Embryo Engineering,College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin 130062) |
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| Abstract: | Objective] The aim was to generate transgenic pig specifically expressing Cre recombinase controlled by PIWIL1 promoter and make basic contribution for research on piRNA and its related genes’ function and mechanism.Method] The promoter of pig PIWIL1 gene was amplified by PCR method.Replace the MHC promoter of pig specific Cre recombinase expression plasmid(pET28a-MHC-Cre-BGHpo1yA-FRT2neor) with PIWIL1 promoter and specific Cre recombinase plasmid(pPIWIL1-Cre) was obtained.Transfect the plasmid to mini-pig fetal fibroblast cells and screen for positive clones with drug and do nuclear transfer.Result] Two transgenic pigs of specific expressing Cre recombinase controlled by PIWILI promoter were got.Conclusion] We generate transgenic pigs that specifically express Cre recombinase successfully and make possible tools for piRNA and related gene function and mechanism research. |
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| Keywords: | piRNA PIWIL1 Cre enzyme Transgenic swine |
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