干扰素通过上调BAX蛋白表达诱导肝癌细胞凋亡的研究

目的]探讨干扰素(IFN)体外诱导肝癌细胞凋亡及其相关基因表达的作用。方法]不同浓度的干扰素(IFN)处理肝癌细胞系CBRH-7919细胞不同时间后,光学显微镜下观察其形态学改变,用琼脂糖凝胶电泳检测作用不同时间干扰素对其细胞凋亡的影响,利用细胞免疫组化技术检测不同作用时间下BAX蛋白表达量的变化。结果]干扰素作用肝癌细胞后,可看到较为典型的细胞凋亡形态学变化:细胞体积缩小,变形,细胞膜完整;细胞核固缩,染色质凝集,核碎裂,染色质片段化,凋亡小体形成等。琼脂糖凝胶电泳测得随着干扰素浓度的增高,出现典型的DNA LADDER。随着时间的增加BAX蛋白表达量出现增加,尤以48 h较明显。结论]IFN可以诱导肝癌细胞凋亡,可能是由于IFN上调了BAX蛋白的表达,从而增强了自身对肝癌细胞的凋亡作用。

Experimental Study on Apoptosis of Hepatoma Cells Induced by Interferon through Up-regulating Expression of BAX Protein

Objective] To explore an apoptosis of CBRH-7919 cells induced by INF in vitro and its expression of BAX.Method] The cells line CBRH-7919 was treated with interferon(IFN) with different concentrations at different time,its morphological change was observed under microscopy.The DNA ladder of cell apoptosis was detected by agarose gel electrophoresis.The expression changes of BAX genes were detected by immunohistochemical method under interferon(IFN) treatment with different concentrations at different time.Result] After IFN treatment,the typical apoptosis was characterized by a decrease in cell volume,condensation nuclei,chromatin condensation,nuclear fragmentation,chromatin fragmentation,formation of apoptotic bodies,etc.The result of agarose gel electrophoresis showed that typical DNA LADDER appeared with the increasing interferon concentrations.The expression of BAX gene increased obviously in 48 hour.Conclusion] IFN could induce the apoptosis of CBRH-7919 cells,which might be resulted from the reason that IFN upregulated the expression of BAX genes and enhanced its role in the apoptosis of hepatoma cells.