| 桑椹红色素不同测定方法的比较研究 |
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| 引用本文： | 唐迪,朱毛毛,赵婷,张敏,顾晓云,仰榴青.桑椹红色素不同测定方法的比较研究[J].安徽农业科学,2012,40(9):5207-5208,5531. |
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| 作者姓名： | 唐迪 朱毛毛 赵婷 张敏 顾晓云 仰榴青 |
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| 作者单位： | 江苏农林职业技术学院,江苏镇江,212400;江苏大学食品与生物工程学院,江苏镇江,212013;江苏大学化学化工学院,江苏镇江,212013 |
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| 摘 要： | 目的]研究和比较桑椹红色素的不同定量分析方法。方法]以酸性乙醇溶液提取桑椹红色素,并用大孔树脂对其进行初步纯化。分别利用色价法、消光系数法、pH示差法和NaNO2-Al(NO3)3比色法测定桑椹花青素的含量。结果]纯化后色素的色价是纯化前的14.8倍;消光系数法测得桑椹红色素中花青素含量为0.015%;pH示差法测得其含量为0.017%;比色法测得含量为0.007 6%。结论]pH示差法测定桑椹花青素含量较为准确。
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| 关 键 词： | 桑椹红色素 提取纯化 定量分析 |
Comparative Study on the Different Quantitative Analysis of Mulberry Red Pigment |
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| Institution: | TANG Di et al(Jiangsu Polytechnic College of Agriculture and Forestry,Zhenjiang,Jiangsu 212400) |
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| Abstract: | Objective] The aim was to study the quantitative analysis of mulberry red pigment.Method] Mulberry red pigment was extracted by using acid alcohol and macroreticular resin was purified.The pigment content was analyzed by color value method,extinction coefficient method,pH differential method and NaNO2-Al(NO3)3 method,respectively.Result] The purified pigment was 14.8 times the color value of unpurified one.The mulberry red pigment content measured by extinction coefficient method was 0.015%.The one measured by pH differential method was 0.017%.The one measured by NaNO2-Al(NO3)3 method was 0.007 6%.Conclusion] pH differential method was more accurate for the quantitative analysis of mulberry anthocyanins. |
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| Keywords: | Mulberry red pigment Extraction and purification Quantitative analysis |
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