超声辅助提取参薯叶黄酮的工艺研究

目的]研究参薯叶黄酮的超声辅助提取工艺条件。方法]以参薯叶为原料,选取提取温度、提取时间、超声波功率和料液比4个因素进行单因素试验,并在单因素试验基础上进行L9(34)正交试验,确定参薯叶黄酮的最佳超声提取工艺条件。结果]优选的最佳提取工艺条件为:乙醇浓度60%,超声提取时间50 min,提取温度65℃,料液比为1∶30(g/ml);在此条件下,薯叶黄酮的提取率为5.17%。结论]该方法优选出了薯叶黄酮的最佳超声提取工艺条件,为参薯叶资源的加工利用开辟新的途径。

Ultrasonic-assisted Extraction Technology of Total Flavonoids from the Leaves of Dioscorea alata L.

Objective] To study on the ultrasonic-assisted extraction technology of flavonoids from the leaves of Dioscorea alata L..Methods] Taking the leaves of D.alata as the raw materials,single factor test of extraction temperature,extraction time,ultrasonic power and solid-liquid ratio was conducted.Based on this,L9(34)orthogonal test was conducted in order to determine the optimal ultrasonic-assisted extraction technology for the flavonoids from D.alata leaves.Results] The optimal extraction technology was as follows:60% ethanol,50 min extraction time,65 ℃ extraction temperature,and 1∶ 30(g/ml)solid-liquid ratio.Under this condition,the extraction rate of flavonoids from D.alata leaves was 5.17%.Conclusion] The optimal ultrasonic-assisted extraction technology of flavonoids from D.alata leaves was selected,which provided new approach for the processing and utilization of D.alata leaves.