霍山产3种石斛多糖含量变化规律

目的]研究霍山产3种石斛多糖含量变化规律。方法]采用硫酸-苯酚法测定霍山石斛、霍山铁皮石斛和霍山细茎石斛3种霍山产石斛在8个不同生长阶段的多糖含量,并分析了其变化的规律和影响因素。结果]在组培苗繁育阶段,石斛多糖含量在相对较低的水平上稳步增长,且呈现出霍山铁皮石斛霍山石斛霍山细茎石斛的规律;石斛在栽培2年后多糖含量达到最高,品种间的规律表现为霍山石斛霍山铁皮石斛细茎石斛,其中霍山石斛2年生茎多糖含量高达35.74%,在3种石斛中是最高的。结论]试验研究了霍山产3种石斛多糖含量变化规律,为石斛的繁育、栽培以及应用提供了依据。

The Content Change of Polysaccharides in 3 Huoshan Species of Dendrobiumn with the Variation of Time

Objective] To study the content change of polysaccharides in 3 Huoshan species of Dendrobiumn with the variation of time.Method] The content of polysaccharides in 3 Huoshan species of Dendrobiumn including Huoshan D.officinale,D.huoshanense and D.moniliforme at 8 growth stages was determined by sulfuric acid-phenol method.And,the changing trend and impacting factors were also analyzed.Result] The content of polysaccharides was increasing steadily based on a low level at the stage of tissue culture,and it decreased in the following order,D.officinale,D.huoshanense,D.moniliforme.The highest content of polysaccharides in those Dendrobium was pursued after two years culture,the order was D.huoshanense > D.officinale >D.moniliforme,among which the content of polysaccharides in D.huoshanense was as high as 35.74%.Conclusion] Those results lay a foundation for the breeding,cultivation and application of those three kinds of Dendrobium.