菊芋生料联合生物加工发酵生产燃料乙醇

目的]在联合生物加工技术发酵菊芋生料生成乙醇工艺中,降低高浓度菊芋生料醪液的黏度,使菊芋发酵乙醇浓度达到或超过目前玉米乙醇的行业水平。方法]利用马克斯克鲁维酵母(Kluyveromyces marxianus),通过添加辅助酶制剂、改变拌料水温及优化补料方式等方法,降低发酵醪液黏度,提高菊芋生料的浓度,从而提高发酵终点的乙醇浓度并缩短发酵时间。结果]最佳工艺条件为:45℃水拌料,pH 5.0,添加0.10%酒精复合酶,发酵初始干粉浓度为240 g/L,分别在发酵12和24 h补料,菊芋干粉终浓度可达到300 g/L,发酵时间48 h,乙醇浓度达到91.6 g/L,乙醇对糖的得率为0.464,为理论值的90.6%。结论]此工艺为菊芋乙醇工业化的生产提供了有利条件。

Production of Fuel Ethanol from Uncooked Helianthus tuberosus by Consolidated Bio-processing (CBP) Fermentation

Objective] During the process of producing ethanol from uncooked Helianthus tuberosus by consolidated bio-processing(CBP) fermentation,the viscosity of high-concentration uncooked Helianthus tuberosus wort was decreased,which could reach or exceed the current industry level of corn ethanol.Method] By using Kluyveromyces marxianus,through adding assistant enzyme,changing water temperature and optimizing fed-batch method,the wort viscosity was decreased and concentration of uncooked Helianthus tuberosus was improved,and then,the final fermentation ethanol concentration was increased and fermentation time was reduced.Result] The optimum processing conditions were: temperature of water 45 ℃,initial pH 5.0,adding 0.10% alcohol compound enzymes,fed-batched at 12 h and 24 h,initial concentration of dry powder was 240 g/L,the final concentration reached 300 g/L,the final ethanol concentration was 91.6 g/L and ethanol yield was obtained 0.464 for the theoretical value of 90.6% at 48 h.Conclusion] The process will provide favorable conditions for industrialization production of Helianthus tuberosus ethanol.