Cd~(2+)处理下超氧阴离子与花生萌发的关系

目的]阐明Cd2+对花生(Arachis hypogaea L.)种子萌发的作用以及花生萌发过程中超氧阴离子(O2-.)含量的变化与酯酶同工酶、淀粉同工酶的关系。方法]分别采用组织化学定位和电泳技术。结果]10、30μmol/L Cd2+处理对花生胚根和下胚轴有显著的促进生长作用,使花生子叶中酯酶同工酶和淀粉同工酶活性增强,子叶以及胚根O2-.含量提高。结论]酯酶同工酶和淀粉同工酶活性的提高与Cd2+诱导的超氧阴离子的提高相关。

Relationship of Superoxide Anion and Ger mination of Peanut (Arachis hypogaea L.)under Treatment of Cadmium

Objective] The aim was to illustrate the relationship between superoxide anion contents and the activities of amylase,esterase isozymes during peanut ger mination under the treatment of Cd2+.Method] Histochemical method and eletrophoresis were applied,respectively.Result] 10,30 μmol/L Cd2+ significantly promoted the growth of radicles and hypocotyls during peanut germination.The activities of esterase and amylase isozymes significantly increases in coty ledons and the superoxide contents in hypocotyls and cotyledons increased under the treatments of 10,30 μmol/L Cd2+.Conclusion] The increase of esterase,amylase isozyme activities linked to the increase of superoxide anion induced by Cd2+.