YB菌株生化需氧量的测定及对河豚毒素毒性的响应

[目的]了解自有YB菌株在BOD生物传感器及河豚毒素毒性传感器中的作用。[方法]以YB菌株作为响应菌株,采用夹层膜法制作固定化微生物膜,组装成微生物传感器并测试其性能。[结果]YB菌株在33℃,pH为7.2的条件下对BOD响应良好,在此条件下静态法测得传感器标准曲线为y=0.061 2x-0.001 3,线性相关系数为0.995;动态法测得传感器标准曲线为y=0.072 2x-0.031 9,线性相关系数为0.996。同时,YB菌株对河豚毒素基本没有响应。[结论]自有YB菌株可以作为BOD微生物传感器的敏感菌,但不能作为河豚毒素毒性传感器测试菌株。

The Determination of BOD and TTX using the YB Bacterial Strain

[Objective] The aim was to learn the function of YB strain in BOD biological sensor and toxin in dolphin.[Method]Considering YB strain as responding strain,and using membrane to fix microbiological membrane,the microbiological sensor was assembled and tested.[Result]When the determination of YB strain on BOD was at 33 ℃ and a pH of 7.2,its response to BOD was good.Under such condition,standard curve of sensor by static method was y=0.061 2x-0.001 3.Linear relevant coefficient was 0.995.Standard curve of sensor by dynamic method was y=0.072 2x-0.031 9.Linear relevant coefficient was 0.996.In the meantime,YB strain didn’t response to toxin in dolphin.[Conclusion]YB strain can be considered as BOD micro biological sensor.However,it can’t test strain as toxin sensor of dolphin.