| 巴西橡胶树HbTCTP基因的克隆及表达 |
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| 引用本文： | 梁小莲,李辉亮,彭世清.巴西橡胶树HbTCTP基因的克隆及表达[J].分子植物育种,2009,7(1). |
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| 作者姓名： | 梁小莲 李辉亮 彭世清 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物牛物技术重点开放实验室,海口,571101;海南省通什农业学校,五指山,572200
2. 中国热带农业科学院热带生物技术研究所,农业部热带作物牛物技术重点开放实验室,海口,571101 |
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| 基金项目： | 国家自然科学基金，中央级公益性科研院所基本科研业务费专项基金，高等学校博士学科点专项科研基金 |
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| 摘 要： | 在先前的研究中通过抑制缩减杂交获得了一个在巴西橡胶幼态无性系与老态无性系胶乳中差异表达的片段(HbSSHl2),BlastX分析表明,由该片段编码的氨基酸与麻疯树(Jatropha curcas L.)翻译控制肿瘤蛋白(translationally controlled tumor protein,TCTP)的同源性达到92%.本研究根据HbSSHl2的序列信息设计引物,通过3L-RACE的方法获得了一个长832bp的cDNA(命名为HbTCVP),序列分析表明HbTCTP有507 bp的阅读框,68 bp的5'-UTR和255 bp的3'UTR,编码168个氨基酸,该氨基酸序列与麻疯树、油棕、花生、南瓜、番茄和拟南芥的TCTP同源性分别达到93.45%、90.48%、89.29%、85.12%、82.74%和79.76%.RT-PCR表明,HbTCTP在巴两橡胶叶片、胶乳及树皮中都有表达,乙烯利处理可诱导HbTCTP的表达,割胶抑制HbTCTP的表达,表明HbTCTP可能参与伤信号或乙烯信号传导.HbTCTP的表达分析有助于解析橡胶树幼态无性系高产的分子机制.
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| 关 键 词： | 乙烯 表达 巴西橡胶树 翻译控制肿瘤蛋白基因 |
Cloning and Expression of Hb TCTP from Hevea brasiliensis |
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| Liang Xiaolian,Li Huiliang,Peng Shiqing.Cloning and Expression of Hb TCTP from Hevea brasiliensis[J].Molecular Plant Breeding,2009,7(1). |
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| Authors: | Liang Xiaolian Li Huiliang Peng Shiqing |
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| Institution: | Liang Xiaolian 1,2 Li Huiliang 1 Peng Shiqing 1 1 Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Biosciences , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101,2 Tongza Agricutural School,Wuzhishan,572200 |
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| Abstract: | By using the information about the sequence from a differentially expressed clone (designated as Hb-SSH12) encodes a translationally controlled tumor protein (TCTP) that was cloned recently by the subtractive hy-bridization between in latex of Hevea brasiliensis juvenile clone and in those of Hevea brasiliensis mature clone, a full-length cDNA encoding TCTP, designated as Hb TCTP, was isolated and characterized from rubber tree. Se-quence analysis revealed that the ORFs of Hb TCTP encode 168 amino acid resi... |
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| Keywords: | Ethylene Expression Hevea brasiliensis Hb TCTP |
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