| 水提大孔吸附树脂柱色谱法制备桔梗总皂苷 |
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| 引用本文： | 杨献文,刘墨祥,刘桂艳,李苏梅,杨全.水提大孔吸附树脂柱色谱法制备桔梗总皂苷[J].扬州大学学报(农业与生命科学版),2002,23(1):15-17. |
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| 作者姓名： | 杨献文 刘墨祥 刘桂艳 李苏梅 杨全 |
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| 作者单位： | 1. 吉林农业大学中药材学院,吉林长春,130118
2. 扬州大学医学院药学系,江苏扬州,225001 |
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| 基金项目： | 国家“九五”重点科技攻关项目 (9690 3 0 2 ) |
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| 摘 要： | 系统研究了影响水提取桔梗总皂苷的各种因素 ,据此建立水提大孔吸附树脂柱色谱制备桔梗总皂苷的方法 ,即 :桔梗粉末 (840 μm)共加 2 0倍量水提取 3次 ,每次 0 .5h ,滤液行大孔吸附树脂柱 ,用水 乙醇溶剂系统洗脱 ,分离得到桔梗总皂苷 ,结果平均得率为 1 .60 3 %。该法操作简单、快速且成本低 ,适于工业化生产。
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| 关 键 词： | 桔梗 桔梗皂苷 大孔吸附树脂 |
| 文章编号： | 1671-4652(2002)01-0015-03 |
| 修稿时间： | 2001年11月21 |
PREPARING PLATYCODINS BY WATER-EXTRACTINGAND MACRORETICULAR RESIN COLUMNCHROMATOGRAPHY-SEPARATING |
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| YANG Xian\|wen ,LIU Mo\|xiang ,LIU Gui\|yan ,LI Su\|mei ,YANG Quan.PREPARING PLATYCODINS BY WATER-EXTRACTINGAND MACRORETICULAR RESIN COLUMNCHROMATOGRAPHY-SEPARATING[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2002,23(1):15-17. |
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| Authors: | YANG Xian\|wen LIU Mo\|xiang LIU Gui\|yan LI Su\|mei YANG Quan |
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| Institution: | YANG Xian\|wen 1,LIU Mo\|xiang 2,LIU Gui\|yan 1,LI Su\|mei 1,YANG Quan 1 |
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| Abstract: | Influential factors on extracting platycodins with water were investigated systematically, on which the method was based for preparing platycodins by water\|extracting and macroreticular resin column chromatography\|separating. That is, to the powder of Radix platycodonis , added altogether 20\|fold water (W/W) and refluxed three times with 0.5 hour for each time. The filtrate was then passed through the macroreticular resin and platycodins were obtained by gradient eluation using water and alcohol as elution system. The average gaining rate of platycodins was 1.603 %. The method offers a basis of industrialized production of platycodins for its advantages of easy, rapid operation, and low cost. |
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| Keywords: | Radix platycodonis Platycodon grandiflorum platycodin macroreticular resin |
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