钙对盐胁迫下黄瓜幼苗生长及可溶性蛋白质表达的影响

以盐敏感型黄瓜品种津春2号为试验材料,在营养液栽培条件下,研究了叶面喷施Ca2+对盐胁迫下黄瓜幼苗生长和可溶性蛋白质表达的影响。结果表明:75 mmol/L NaCl胁迫下植株生物量显著下降(P<0.05),叶面喷施4 mmol/L CaCl2可显著提高盐胁迫下黄瓜幼苗的生物量(P<0.05)。在盐胁迫处理下,黄瓜幼苗叶片可溶性蛋白质含量显著下降(P<0.05),喷施Ca2+第3 d时黄瓜幼苗叶片中可溶性蛋白质含量降低(P<0.05),而第6 d和9 d时升高(P<0.05),黄瓜幼苗根系可溶性蛋白质含量先升高后降低(P<0.05),喷施Ca2+后黄瓜幼苗根系可溶性蛋白质含量先降低后升高(P<0.05)。SDS-PAGE的分析结果表明,盐胁迫下黄瓜幼苗叶片中至少有12种蛋白质表达量发生明显变化,其相对分子量分别为:8.75×104、7.79×104、7.57×104、7.41×104、6.51×104、5.43×104、5.05×104、4.47×104、4.07×104、3.14×104、2.60×104和2.15×104;根系中至少有7种蛋白质表达量发生变化,其相对分子量分别为:8.11×104、7.66×104、4.69×104、4.52×104、3.95×104、2.38×104和1.68×104。

Effects of calcium on growth and expression of soluble protein in cucumber seedlings under salt stress

A hydroponic experiment was conducted to study the effects of exogenous calcium on growth and soluble protein expression of salt sensitive cucumber cultivar Jinchun 2(Cucumis sativus L.) under salt stress.Exogenous Ca2+ significantly increased the biomass under 75 mmol/L NaCl stress(P<0.05).The soluble protein content in cucumber leaf significantly was decreased by salt stress(P<0.05),however,it was improved at sixth day of Ca2+ application(P<0.05).The soluble protein content of cucumber root was increased first and then decreased under salt stress(P<0.05),however it was decreased first and then increased after Ca2+ application(P<0.05).The results of SDS-PAGE analysis showed that the expressions of twelve proteins with relative molecular weights of 8.75×104,7.79×104,7.57×104,7.41×104,6.51×104,5.43×104,5.05×104,4.47×104,4.07×104,3.14×104,2.60×104 and 2.15×104 changed obviously in cucumber leaves,and seven proteins with relative molecular weights of 8.11×104,7.66×104,4.69×104,4.52×104,3.95×104,2.38 ×104 and 1.68×104 in roots changed in roots under salt stress as well.