| 长顺绿壳蛋鸡体尺性状与IGF1基因相关性研究 |
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| 引用本文： | 韦雄,李家兴,任丽群,刘祥林,吴泓友,孙鹃,陈晶,陈祥. 长顺绿壳蛋鸡体尺性状与IGF1基因相关性研究[J]. 江苏农业学报, 2012, 28(4): 804-808 |
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| 作者姓名： | 韦雄 李家兴 任丽群 刘祥林 吴泓友 孙鹃 陈晶 陈祥 |
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| 作者单位： | 1. 高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州大学动物科学学院,贵州贵阳550025
2. 贵州省畜牧技术推广站,贵州贵阳,550001 |
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| 基金项目： | 贵州省动植物育种专项，贵州大学大学生SRT项目 |
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| 摘 要： | 采用PCR-SSCP技术和DNA测序技术对124只贵州长顺绿壳蛋鸡的胰岛素生长因子1基因(IGF1)的In3片段进行多态性分析,并与体尺性状进行关联性研究,为贵州绿壳蛋鸡的遗传评估提供资料,并为选育提供有育种价值的分子标记。结果表明:试验鸡群的IGF1基因In3片段存在3个单核苷酸突变位点,分别为A(-981)G、A(-953)T和T(-881)C;该群体偏离Hardy-Weinberg平衡状态,且该位点处于高度多态(PIC>0.5);F2F2基因型个体体斜长和胸深均低于其他基因型个体,推测该基因型可能是长顺绿壳蛋鸡向小型蛋鸡方向选育的有效分子标记。
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| 关 键 词： | IGF1基因 绿壳蛋鸡 体尺性状 |
Association of IGF1 gene with body traits of Changshun blue-shell chickens |
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| WEI Xiong , LI Jia-xing , REN Li-qun , LIU Xiang-lin , WU Hong-you , SUN Juan , CHEN Jing , CHEN Xiang. Association of IGF1 gene with body traits of Changshun blue-shell chickens[J]. Jiangsu Journal of Agricultural Sciences, 2012, 28(4): 804-808 |
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| Authors: | WEI Xiong LI Jia-xing REN Li-qun LIU Xiang-lin WU Hong-you SUN Juan CHEN Jing CHEN Xiang |
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| Affiliation: | 1(1.Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education/College of Animal Sciences,Guizhou University,Guiyang 550025,China;2.Guizhou Provincial Livestock Bureau,Guiyang 550001,China) |
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| Abstract: | In order to provide genetic evolution data and molecular markers for Guizhou blue-shell chickens breeding,PCR-SSCP and DNA sequencing technology were used to analyze the polymorphism and association of insulin-like growth factor(IGF1) gene on In3 fragment with body traits from 124 Changshun blue-shell chickens.The results showed that there were three nucleotide mutations,A(-981) G,A(-953) T and T(-141) C,in In3 fragment of IGF1 gene and the test groups were in high polymorphic state(PIC>0.5).The distributions of genotypes were deviated from Hardy-Weinberg equilibrium(P<0.05).Association analysis indicated that the body slanting length and chest depth of the chickens with F2F2 genotype were both lower than those with other genotypes,and the somatotype with F2F2 genotype was relatively small,suggesting that genotype F2F2 might be the marker for the breeding of Changshun blue-shell chickens towards miniature laying chickens. |
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| Keywords: | IGF1 gene blue-shell chicken body trait |
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