| 中国林蛙皮多糖类天然保湿因子的提取与鉴定 |
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| 引用本文： | 王大为,张艳荣,沙坤. 中国林蛙皮多糖类天然保湿因子的提取与鉴定[J]. 吉林农业大学学报, 2002, 24(6): 99-102,106 |
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| 作者姓名： | 王大为 张艳荣 沙坤 |
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| 作者单位： | 吉林农业大学食品工程学院,吉林,长春,130118 |
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| 基金项目： | 吉林市科委资助项目[吉市科农字第(2001)19号] |
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| 摘 要： | 采用复合酶解法从中国林蛙皮中分离提取出天然保湿因子，并采用红外吸收光谱法对天然保湿因子进行了定性测试，采用卟唑（carbazole)法和Elson-Morgan法对天然保温因子的含量及纯度进行了测定。结果表明：采用本研究方法分离、提纯的天然保湿因子为透明质酸（Hyaluronic acid,HA)，其外观呈白色粉末,葡萄醛酸含量43．2％，氨基葡糖含量39．7％，无臭、无味、无毒，具有强保湿性，可应用于保健食品、化妆品及眼科药和。
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| 关 键 词： | 中国林蛙皮 多糖类 天然保湿因子 含量 纯度 |
| 文章编号： | 1000-5684(2002)06-0099-04 |
Extraction and Identification of Natural Moist Factor from Rana tempoviductus Ranaearia Chensinensis David Skin |
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| WANG Da-wei,ZHANG Yan-rong,SHA Kun. Extraction and Identification of Natural Moist Factor from Rana tempoviductus Ranaearia Chensinensis David Skin[J]. Journal of Jilin Agricultural University, 2002, 24(6): 99-102,106 |
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| Authors: | WANG Da-wei ZHANG Yan-rong SHA Kun |
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| Abstract: | Natural moist factor was extracted from Rana-Skin using composition enzymolysis method, and its nature, content and purity were determined using infrared ray absorption method, and Carbazole method and Elson-Morgan method.The result shows that this natural moist factor is hyaluronic acid(HA) . It is white powder,smelless,poisonless,and strong in keeping moisture. It contains 43.2% of glucuronic acid, and 39.7 % of amino-glucose. It can be used in health food, cosmetics keeping moisture and ophthalmo-logical medicine. |
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| Keywords: | Rana tempoviductus ranaearia chensinensis david skin moist factor content purity iden- tification |
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