Introduction of T-DNA into Watermelon by Stigma Smeared Method and Its Molecular Marker Research

Objective]The aim was to introduce T-DNA into watermelon for its molecular marker research.Method]Based on the method of foreign DNA introduced to Arabidopsis thaliana via dipping flowers,the stigma smear was used to transfer T-DNA into watermelon and its molecular marker research was carried out.Result]The ideal transformed species was ZXG01078 for the highest fruit setting rate and the most deviant seedlings.The best concentration of kanamycin for treating watermelon seeds was 500-700 mg/L with differences among the species.The best position was spire leaf or young leaf and the best concentration of kanamycin for treating the watermelon leaf was 4 000-8 000 mg/L with no significant difference among species.The steadily variation appearing of growing pointless and conjoined twin seedlings indicated that the normal growth had been interfered by foreign DNA in the progeny.Conclusion]This study had provided basis for the further research on watermelon.