| 反相高效液相色谱法测定金银花中黄酮类成分的含量 |
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| 引用本文： | 方传代,邹盛勤.反相高效液相色谱法测定金银花中黄酮类成分的含量[J].安徽农业科学,2006,34(11):2321-2322. |
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| 作者姓名： | 方传代 邹盛勤 |
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| 作者单位： | 宜春学院理工学院,江西,宜春,336000;江西省天然药物活性成分研究重点实验室,宜春学院生物工程研究所,江西,宜春,336000 |
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| 摘 要： | 利用反相高效液相色谱法,配以二极管阵列检测器测定了金银花中芦丁和槲皮素的含量,建立了一种金银花的质量控制方法。色谱柱为Nova-Pak C18柱,流动相为甲醇和0.3%H3PO4(50∶50,体积比),流速0.6 ml/min,检测波长256 nm,柱温25℃。芦丁和槲皮素的平均回收率分别为97.9%和96.3%,RSD分别为1.5%和1.3%。该方法简单、灵敏、结果准确、重现性好,可同时分析金银花中2种黄酮类成分。
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| 关 键 词： | 金银花 黄酮 芦丁 槲皮素 反相高效液相色谱法 |
| 文章编号： | 0517-6611（2006）11-2321-02 |
| 收稿时间： | 2006-03-21 |
| 修稿时间： | 2006-03-21 |
Study on RP-I-IPLC Method for Determination of Two Flavone Compounds of Flos lonicerce |
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| FANG Chuan-dai et al.Study on RP-I-IPLC Method for Determination of Two Flavone Compounds of Flos lonicerce[J].Journal of Anhui Agricultural Sciences,2006,34(11):2321-2322. |
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| Authors: | FANG Chuan-dai |
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| Institution: | Institute Technology, Yichun University, Yichun,Jiangxi 336000 |
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| Abstract: | A reversed phase high performance liquid chromatographic method with photodiode array detection was applied for the quality control of Flos lonicerae.Rutin and quercetin in Flos lonicerae were separated in a Nova-Pak C18 column with methanol-0.3% H_3PO_4(50∶50,v/v) as mobile phase at the flow rate of 0.6 ml/min.The column temperature was controlled at 25 ℃ and the wavelength of detector was set at 256 nm.The average recoveries of rutin and quercetin were 97.9% and 96.3%.RSD were 1.5% and 1.3%,respectively.The method was simple,sensitive,precise and reproducible,and can be used to determine two flavonoid components of Flos lonicerae. |
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| Keywords: | Flos lonicerae Flavonoid Rutin Quercetin Reversed phase high performance liquid chromatographic |
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