| 应用RAPD方法获得与番茄ToMV抗性基因Tm2nv连锁的分子标记 |
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| 引用本文： | 田苗英,冯兰香.应用RAPD方法获得与番茄ToMV抗性基因Tm2nv连锁的分子标记[J].植物病理学报,2000,30(2):158-161. |
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| 作者姓名： | 田苗英 冯兰香 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所, 北京 100081 |
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| 基金项目： | ARC-AVRDC和植物病虫害生物学国家重点实验室开放基金资助项目 |
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| 摘 要： | 运用RAPD技术,在番茄ToMV抗性基因Tm2nv的F2代群体中采用混合分组分析法(bulkedse gregant analysis,BSA)进行分子标记研究,找到了一个与Tm2nv基因连锁的分子标记OPD201700,其遗传距离为7.067cM,LOD值为16.768。
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| 关 键 词： | 番茄 番茄花叶病毒 随机扩增多态性DNA 分子标记 |
IDENTIFICATION OF A MOLECULAR MARKER LINKED TO ToMV RESISTANCE GENE Tm2nv IN TOMATO USING RANDOMLY AMPLIFIED POLYMORPHIC DNA |
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| TIAN Miao-ying,FENG Lan-xiang,YANG Cui-rong,GONG Hui-zhi.IDENTIFICATION OF A MOLECULAR MARKER LINKED TO ToMV RESISTANCE GENE Tm2nv IN TOMATO USING RANDOMLY AMPLIFIED POLYMORPHIC DNA[J].Acta Phytopathologica Sinica,2000,30(2):158-161. |
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| Authors: | TIAN Miao-ying FENG Lan-xiang YANG Cui-rong GONG Hui-zhi |
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| Institution: | Institute of Vegetables and Flowers, CAAS, Beijing 100081, China |
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| Abstract: | Randomly amplified polymorphic DNA (RAPD) was employed as a tool to detect molecular markers linked to the ToMV resistance gene Tm2nv in tomato. A specific DNA band OPD201700 was proved to be linked to the gene Tm2nv by bulked segregating analysis (BSA) with F2 population. The genetic distance was measured as 7.067cM and LOD was 16.768. |
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| Keywords: | tomato ToMV RAPD molecular marker |
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