拮抗酵母BS-316抑菌成分分离与应用的初步研究

以拮抗酵母菌BS-316为试材,研究酵母菌及其培养液对灰霉菌的抑菌性,用柱层析的方法对酵母培养液进一步分离纯化,并测定了粗提纯组分对灰霉菌的抑菌性。结果表明,酵母菌及其培养液对灰霉菌均有一定的抑制作用,酵母菌悬液对灰霉菌的抑菌性高于酵母培养液;酵母培养液经粗提取后,随着接种时间的延长,粗提纯组分对灰霉菌的抑制作用高于培养原液;活体试验中,粗提纯组分对番茄果实灰霉病的扩展有明显的抑制作用,其病斑直径显著小于酵母培养液处理(P0.05)。表明拮抗酵母菌BS-316除了细胞抑菌外,还可能产生了某些抑菌物质存在于培养液中,并且经纯化后可能用于果实灰霉病的防治保鲜。

Preliminary Study on Isolation and Application of Antimicrobial Components from Antagonistic Yeast BS-316

The antibacterial activity of antagonistic yeast BS-316 and its cultural liquid on Botrytis cinerea was studied, separation and purification of yeast culture medium by column chromatography were carried out and the antimicrobial activity of crude purified components against Botrytis cinerea was also determined. The result showed that both the yeast cell of BS-316 and its cultural liquid had antibacterial effects on Botrytis cinerea, and the bacteriostatic activity of yeast suspension was higher than yeast culture. With the extension of inoculation time, the inhibitory effect of the crude purified components was higher than the culture medium. The crude purified components could inhibit the expansion of tomato fruit grey mold, and the diameter of disease spot was significantly smaller than that of culture medium. In vitro experiment, it suggested that BS-316 could produce some inhibition substance in the medium, which could be used to prevent and control the gray mold of postharvest fruits after purifying.