| 转基因棉子油的快速PCR检测研究 |
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| 引用本文： | 袁建琴,唐中伟,许冬梅,刘建东,高斌战. 转基因棉子油的快速PCR检测研究[J]. 棉花学报, 2009, 21(2): 153-155 |
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| 作者姓名： | 袁建琴 唐中伟 许冬梅 刘建东 高斌战 |
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| 作者单位： | 山西农业大学生命科学学院,山西,太谷030801;山西隆克尔生物制药有限公司,山西,太谷030800 |
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| 基金项目： | 农业部第五批部级质检中心筹建计划，山西农业大学科技创新项目 |
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| 摘 要： | 以棉子油为原料，探索出了一种快速、简便的转基因棉子油的PCR检测方法。该方法能从15 mL棉子油中提取0.4 μg高纯度DNA，用于PCR检测。针对18S rDNA、CaMV35S、NOS、NPTⅡ和Cry1A（c）等基因进行了棉子油中内外源基因PCR检测，分别扩增出不同的目的片段。本研究为油脂类产品的外源基因检测提供了可行方法。
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| 关 键 词： | 棉子油 转基因成分 DNA提取 PCR 检测研究 |
Study of Rapid Detection of Transgene Sequences in Cottonseed Oil Using PCR |
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| YUAN Jian-qin,TANG Zhong-wei,XU Dong-mei,LIU Jian-dong,GAO Bin-zhan. Study of Rapid Detection of Transgene Sequences in Cottonseed Oil Using PCR[J]. Cotton Science, 2009, 21(2): 153-155 |
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| Authors: | YUAN Jian-qin TANG Zhong-wei XU Dong-mei LIU Jian-dong GAO Bin-zhan |
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| Affiliation: | 1.College of Life Science;Shanxi Agricultural University;Taigu;Shanxi 030801;China;2.Lucker Pharmaceutical Stock Factory;Shanxi 030800;China |
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| Abstract: | A simple and rapid-method which could extract DNA from cottonseed oil had been developed. A yield of 0.4 μg DNA could be obtained from 15 mL oil. PCR amplification of sequences of CaMV35S, NOS, NPTⅡ,Cry1A（c）, and an endogenous reference gene 18S rDNA in refined cottonseed oil was performed. Results showed that the sequence-specific fragments were amplified in the samples, demonstrating that the method could be applied for detection of transgene sequences. There are some DNA fragments in refined cottonseed oil, and PCR amplification can detect the foreign DNA. |
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| Keywords: | PCR |
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