三种城市景观竹土壤微生物群落结构特征

为探究城市景观竹林对土壤微生物群落的影响，选取上海辰山植物园种植的3种景观竹，利用Illumina MiSeq高通量测序结合土壤理化指标分析研究了不同景观竹林对土壤理化性质和微生物群落结构特征的影响。结果表明：不同竹林土壤全磷、有效磷、有机质、全氮、全钾含量和电导率存在显著差异(P<0.05)；土壤细菌丰富度和多样性指数在毛竹林土壤中最高，在淡竹林土壤中最低；土壤细菌优势门为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)和绿弯菌门(Chloroflexi)，真菌优势门为子囊菌门(Ascomycota)，其中放线菌门和绿弯菌门在淡竹林土壤中的相对丰度最高，且显著高于其他竹林，而变形菌门、粘球菌门(Myxococcota)的相对丰度在毛竹林土壤中最高，土壤真菌优势门在不同竹林间的差异不显著。此外，土壤细菌群落结构和丰度受土壤p H、硝态氮、电导率、全磷、铵态氮、含水率、速效钾和微生物生物量碳影响显著，而真菌群落变化受全磷、电导率和全钾影响显著。

Soil Microbial Community Structures Under Three Urban Landscape Bamboo Forests

In order to explore the effects of urban landscape bamboo forest on soil microbial community, Illumina MiSeq high-throughput sequencing technology and soil physicochemical indexes were used to analyze the effects of bamboo forests on soil physicochemical properties and microbial community structure in chenshan Shanghai Botanical Garden. It was found the contents of TP, AP, OM, TN, TK and EC in 12 soil physical and chemical indexes were significantly changed with the change of bamboo species, soil bacterial Sobs index and Shannon index of Phyllostachys edulis were the highest, while soil bacterial sobs index and Shannon index of Phyllostachys glauca were the lowest, The dominant phylum of soil bacteria was Actinobacteriota, Proteobacteria, Acidobacteriota and Chloroflexi, while that of fungi was Ascomycota, the relative abundance of Actinobacteriota and Chloroflexi in the soil of Phyllostachys glauca was significantly higher than that of other bamboo forests, relative abundance of Proteobacteria, Myxococcota, Firmicutes and Bacteroidota was the highest in Phyllostachys edulis forest soil. There was no significant difference in dominant phylum of soil fungi among different bamboo forests. In addition, TP, NN, EC, MC, PH were significantly correlated with the distribution of bacterial community, soil factors no significant correlation between fungal community variation at phylum level and selected environmental factors.