| 禽流感病毒新疆株A/Duck/XJ/4的M基因的序列分析 |
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| 引用本文： | 沙依兰古丽,汪萍,成进,巴特力,巴格德力,崔尚金,方新元. 禽流感病毒新疆株A/Duck/XJ/4的M基因的序列分析[J]. 新疆农业科学, 2008, 45(4): 722 |
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| 作者姓名： | 沙依兰古丽 汪萍 成进 巴特力 巴格德力 崔尚金 方新元 |
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| 作者单位： | 新疆畜牧科学院兽医研究所,乌鲁木齐,830000;新疆维吾尔自治区兽医防疫监督总站,乌鲁木齐,830000;中国农业科学院哈尔滨兽医研究所,哈尔滨,150001;米泉县动物防疫监督站,新疆米泉,831400 |
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| 摘 要： | 利用RT-PCR技术对禽流感病毒新疆株A/Duck/XJ/4的M基因进行了扩增与克隆,得到了全长核苷酸序列为1 016 bp的M基因,序列分析表明,M基因最大的开放阅读框位于19～1 000碱基;M1蛋白位于19～777碱基,编码252个氨基酸.M2蛋白位于19～44碱基和733～1 000碱基,编码97个氨基酸.同源性分析显示,A/Duck/XJ/4 株M基因的核苷酸序列和氨基酸序列与所选H5N1亚型的参考毒株以及所选不同HA亚型的参考毒株均有很高的同源性.
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| 关 键 词： | 禽流感病毒新疆株 H5N1 M基因 序列分析 |
| 收稿时间： | 2008-04-25 |
Sequence Analysis of M Gene of Avian Influenza Virus Xinjiang Strain A/Duck/XJ/4 |
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| Shayilanguli,WANG Ping,CHENG Jin,Bateli,Bagedeli,CUI Shang-jin,FANG Xin-yuan. Sequence Analysis of M Gene of Avian Influenza Virus Xinjiang Strain A/Duck/XJ/4[J]. Xinjiang Agricultural Sciences, 2008, 45(4): 722 |
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| Authors: | Shayilanguli WANG Ping CHENG Jin Bateli Bagedeli CUI Shang-jin FANG Xin-yuan |
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| Abstract: | The M gene of A/Duck/XJ/4 strain was amplified by RT-PCR and cloned.The obtained complete sequence of M gene was 1 016 base pairs.Sequence analysis showed that the biggest open reading frame(ORF) was from 19 to 1 000 base pair.M1 protein was located from 19 to 777 base pair and encoded 252 amino acids.M2 protein was located from 19 to 44 base pair and 733～1 000 base pair and encoded 97 amino acids.Homology analysis indicated that M gene of A/Duck/XJ/4 strain,compared with reported H5N1 strains and different HA subtype strains,has high nucleotide Homology and amino acid homology. |
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| Keywords: | H5N1 |
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