| 实时荧光定量PCR检测布鲁菌方法的建立 |
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| 引用本文： | 曹琛福,刘学武,花群义,吕建强,秦智锋,陈兵,曾少灵,孙洁.实时荧光定量PCR检测布鲁菌方法的建立[J].动物医学进展,2009,30(7):6-9. |
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| 作者姓名： | 曹琛福 刘学武 花群义 吕建强 秦智锋 陈兵 曾少灵 孙洁 |
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| 作者单位： | 1. 深圳出入境检验检疫局动植物检验检疫技术中心,广东深圳,518010
2. 上海市崇明县动物疾病预防控制中心,上海崇明,202100 |
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| 基金项目： | 深圳出入境检验检疫局科技项目(SZ2007035) |
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| 摘 要： | 旨在建立实时荧光定量PCR检测布鲁菌的方法。经选取高度保守的具布鲁菌属特异性的BSP31基因设计了一对引物及探针,并采用矩阵法优化反应体系,筛选出引物、探针、dNTP和Mg2+最优浓度配比,同时进行了特异性和灵敏性试验,建立了实时荧光定量PCR检测布鲁菌的方法。该方法可用于对布鲁菌病普查监测及进出境动物及其产品的检验检疫。
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| 关 键 词： | 布鲁菌 实时荧光定量PCR 检测 |
Detection of Brucella by Real-time PCR |
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| CAO Chen-fu,LIU Xue-wu,HUA Qun-yi,L Jiang-qiang,QIN Zhi-feng,CHEN Bing,ZENG Shao-ling,SUN Jie.Detection of Brucella by Real-time PCR[J].Progress In Veterinary Medicine,2009,30(7):6-9. |
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| Authors: | CAO Chen-fu LIU Xue-wu HUA Qun-yi L Jiang-qiang QIN Zhi-feng CHEN Bing ZENG Shao-ling SUN Jie |
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| Institution: | 1.Center of Animal and Plant Inspection & Quarantine of Shenzhen Entry-Exit Inspection and Quarantine Bureau;Shenzhen;Guangdong;518010;China;2.Chongming Center for Animal Disease Control & Prevention;Shanghai;202100;China |
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| Abstract: | This article was purposed of detection of Brucella spp by real-time PCR.A probe and a pair of primers were designed with BSP31 gene of Brucella,which is conversed domain in Brucella spp.The assay was optimized though matrix method,choseing the best concentration of the primers,probe,dNTP and Mg2+.The specificity and sensitivity of the assay were tested.This method can be used for general investigation of brucellosis in agriculture departments,and quarantine of entry-exit animals and their products. |
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| Keywords: | Brucella real-time PCR detection |
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