| MSTN基因内含子2多态性与山羊体重性状相关研究 |
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| 引用本文： | 刘铮铸,李祥龙,巩元芳,靳晓敏,冯敏山. MSTN基因内含子2多态性与山羊体重性状相关研究[J]. 畜牧兽医学报, 2006, 37(8): 745-748 |
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| 作者姓名： | 刘铮铸 李祥龙 巩元芳 靳晓敏 冯敏山 |
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| 作者单位： | 1. 河北科技师范学院动物科学系,昌黎,066600
2. 河北农业大学动物科技学院,保定,071001;河北科技师范学院动物科学系,昌黎,066600 |
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| 基金项目： | 河北省自然科学基金（C2005000241）;河北省教育厅（B2004513）资助 |
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| 摘 要： | 以波尔山羊以及波尔山羊与唐山奶山羊的杂交后代共计102只山羊为材料，根据山羊MSTN基因序列（GenBank登录号：DQ167575）设计一对引物，用PCR—RFLP法进行多态性分析。对该片段测序发现共有2处突变。对突变位点产生的不同基因型与体重进行分析表明：AA型个体的断乳重显著高于BB型和AB型（P〈0.05）。CC型个体的初生重显著高于CD型（P〈0.05）。由此初步推断MSTN基因可能是影响山羊体重性状的主基因或与主基因相连锁，可以用该位点对山羊体重性状进行标记辅助选择。
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| 关 键 词： | 山羊 MSTN 遗传多态性 RFLP 体重 |
| 文章编号： | 0366-6964（2006）08-0745-07 |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006-03-13 |
Relationship between Polymorphism of Goat MSTN Gene Intron 2 and Body Weight |
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| LIU Zheng-zhu,LI Xiang-long,GONG Yuan-fang,JIN Xiao-min,FENG Min-shan. Relationship between Polymorphism of Goat MSTN Gene Intron 2 and Body Weight[J]. Chinese Journal of Animal and Veterinary Sciences, 2006, 37(8): 745-748 |
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| Authors: | LIU Zheng-zhu LI Xiang-long GONG Yuan-fang JIN Xiao-min FENG Min-shan |
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| Abstract: | One pair of primer for goat MSTN gene intron 2 was designed according to goat MSTN gene sequence(Accession No.DQ167575) and PCR-RFLP was carried out in a total number of 102 individuals including Boer goat and its backcrossed offspring to Tangshan dairy goat.The result of sequencing showed that there were two substitution mutations in the fragments.Statistical analysis indicated that AA genotype had significant higher weaning weight than BB and AB genotype(P<0.05).CC genotype had significant higher birth weight than CD genotype(P<0.05).It could be inferred that the MSTN gene maybe a major gene or linked to the major gene affecting the body weight.The polymorphic site could be used to marker assisted selection program for body weight. |
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| Keywords: | goat MSTN genetic polymorphism RFLP body weight |
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