小麦F4籽粒中Glu-1基因的遗传多态性

采用10％SDS—PAGE方法分析JY97—1／blosky和JY97—2／blosky各10个株系100个单株500粒F4籽粒的高分子量麦谷蛋白亚基(HMW—GS)组成，结果表明：1．Glu—D1位点的5 10，5 12的遗传在JY97—1／blosky后代中符合一对相对基因的分离。2．JY97—2／blosky后代HMW—GS组成类型变异极其丰富。在G1u—A1，G1u—B1和G1u—D1三个位点上分别检测到2，9和6种不同的亚基类型，在Glu—A1位点上1亚基和null的频率分别为52．85％和47．15％；Glu—B1位点上的变异最丰富，出现20；7 8 9；6 8等七种新的等位基因类型；Glu—D1位点存在2 5 10；2 12，5 10；5 12等四种新的等位基因类型。最优亚基组合1，7 8，5 10的比例为11．84％。3．两个组合中Glu—B1b或及Glu—B1c的不完全表达率分别为2．07％和1．9％。

Genetic Diversity of Glu-1 Genes in F4 Seeds of Wheat

The composition of high molecular weight glutenin subunit (HMW-GS) of F4 seeds of two crosses (JY97-l/blosky and JY97-2/blosky) were analysis by 10% SDS-PAGE. The results showed that the Glu-D1 subunits (5 + 10,5+10+12.5 + 12) segregation proportion of F4 seeds was 7 : 2 : 7 in cross JY97-l/blosky and the HMW-GS composition types of JY97-2/blosky were extremely abundant. 2, 9 and 6 different HMW-GS types were separately found in Glu-A1, Glu-B1 and Glu-Dl. At the Glu-A1 locus, frequency of subunit 1 and null was 52. 85% and 47. 15%. Variations of HMW-GS at Glu-B1 were the most abundant, there were seven new allelic genes appeared, as subunits 20 and 7 + 8 + 9, 6 +8 etc. At the Glu-D1 locus, there were 2 + 5 + 10, 2 + 12, 5+10, 2 + 5+12 etc. four new allelic genes. The rate of the best of composition of high molecular weight glutenin subunit (1,7 + 8,5 + 12) was 11. 84%. The incomplete expression of rate of Glu-B1b or Glu-B1c was 2. 07% and 1. 9%.