野芥芥酸合成酶基因fae1的克隆与序列分析

根据GenBank中脂肪酸延长酶基因fae1序列(AY 888037)设计了PCR扩增引物,以野芥总DNA为模板进行扩增得到了特异扩增条带.测序结果表明,该片段长1 651 bp,序列分析表明其氨基酸编码区为55～1 575 bp,不含内含子序列,共编码506个氨基酸.该基因序列已提交GenBank,登录号为FJ870905.BLASTn分析结果表明,野芥中fae1基因与其他芸薹属品系的fae1基因核苷酸同源性为76%～98%;BLASTp分析结果表明,野芥中的FAE1与油菜中FAE1存在32个位点差异,其中部分差异可能导致了芥酸含量的不同.

Gene Cloning and Sequence Analysis of Fatty Acid Elongase 1 (fae1) in Sinapis arvensis L.

PCR primers were designed according to the foe1 (Fatty acid elongase 1,fael) DNA sequence of Brassi-ca napus Zhongshuang 9 reported in Gen Bank ( Accession No. AY888037) , PCR was carried out with genomic DNA of Sinapis arvensis as template and the specific fragment was purified and cloned. Result of sequencing showed that the frag-ment was 1 651 bp and analysis of sequence indicated that the coding region was 1 521 bp and encoded 506 amino acids. Results of BLASTn demonstrated that the fael from Sinapis arvensis has 76 % - 98 % nucleotide similarity with genes cloned from Brassica species, and BLASTp demonstrated that 32 amino acids residue substitutions were found between Sinapis arvensis and Brassica species,some sequence changes could explain lines with different erucic acid content.