油桐品种抗黑斑病生理生化机制研究——Ⅲ.品种抗性与多酚氧化酶之关系

在健叶(果皮)上,多酚氧化酶活性、比活性均不反映品种间抗病性的规律性变化,但病叶(果皮)的多酚氧化酶活性、比活性都是抗病品种比感病品种高;分析了离病斑不同距离组织的多酚氧化酶活性及比活性,发现抗病品种在病斑近处酶活、比活大幅度提高,但波及范围较窄,感病品种在病斑近处酶活、比活提高幅度较小,但影响范围较广;病叶多酚氧化酶同工酶抗病品种为2—3条谱带,感病品种仅为1条谱带。

A Study on the Physiological and Biochemical Mechanism of Aleurites fordii Varieties Resisting Black Spot (Ⅲ) The relationship between variety resistance and polyphenol oxidase

The polyphenol oxidase activities (POA) and specific activities (SA)in the healthy leaves and peel do not show the regular changes of disease resis-tance among Aleurites ford ii varieties. In the diseased leaves and peel, however,POA and SA of resistant varieties are higher than those of susceptible ones. Af-ter POA and SA in the tissues at the different distances from spots have beenanalyzed, it is found that near the spots POA and SA of resistant varieties in-crease on a large scale while the range involved is comparatively narrow and nearthe spots POA and SA of susceptible varieties increase just on a smaller scalewhile the range involved is relatively extensive. In the diseased leaves, there are2--3 bands of polyphenol oxidase isozyme for the resistant varieties and only onefor the susceptible varieties.