| SRAP对东北区骨干甜菜单胚品系的遗传多样性分析 |
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| 引用本文： | 李博,王茂芊,王华忠. SRAP对东北区骨干甜菜单胚品系的遗传多样性分析[J]. 中国甜菜, 2012, 0(2): 11-14 |
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| 作者姓名： | 李博 王茂芊 王华忠 |
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| 作者单位： | [1]黑龙江省普通高等学校甜菜遗传育种重点实验室／黑龙江大学农作物研究院,哈尔滨150080 [2]中国农业科学院甜菜研究所,哈尔滨150080 [3]中国农业科学院北方糖料作物资源与利用重点开放实验室,哈尔滨150080 |
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| 基金项目： | 国家现代农业产业技术体系建设项目-国家甜菜产业技术体系建设项目：东北区甜菜育种岗位专家CARS-210104（2011-2015） |
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| 摘 要： | 为了探明东北区甜菜单胚不育系的遗传基础和类群划分,使用SRAP分子标记技术对48份东北区甜菜单胚品系骨干材料的遗传多样性进行分析。筛选出21对多态性较高的引物组合对供试材料进行PCR扩增,共扩增出366条带,其中196条是多态性带,平均多态性条带比率是53.6%。平均遗传距离是0.3945,平均遗传相似系数是0.6740。利用MEGA3.1软件,在遗传距离0.20处,供试材料被分为5个类群。结果表明,东北区48份甜菜单胚品系骨干材料的遗传多样性较为丰富,利用各类不育系做亲本,将可获得杂交优势好的杂交组合。
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| 关 键 词： | 甜菜 SRAP分子标记 单胚品系 遗传多样性 |
Genetic Diversity Analysis of Sugarbeet Monogerm Genotypes in the Northeast Region by SRAP Molecule Marker |
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| LI Bo,WANG Mao-qian,WANG Hua-zhong. Genetic Diversity Analysis of Sugarbeet Monogerm Genotypes in the Northeast Region by SRAP Molecule Marker[J]. , 2012, 0(2): 11-14 |
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| Authors: | LI Bo WANG Mao-qian WANG Hua-zhong |
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| Affiliation: | 1.The Key Laboratory of Sugarbeet Genetic Breeding/Institute for Crop Research,Heilongjiang University,Harbin 150080,China;2.Sugarbeet Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150080,China;3.The Key Laboratory of North Sugar Crop Resource and Utilization,Chinese Academy of Agricultural Sciences,Harbin 150080,China) |
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| Abstract: | SRAP molecule marker was used to test the genetic diversity of 48 monogerm genotypes of sugarbeet in order to research the genetic basis and divide class group of monogerm sterile lines in the northeast of China.21 of 60 pairs primer were obtained on basis of availability.A total 366 unambiguous bands were obtained,196 of total were polymorphic.The average ratio of polymorphic bands was 53.6%,Compute over-all mean showed that genetic distance was 0.3945,genetic similarity coefficient among varieties was 0.6740.The materials were divided into five cluster groups based on cluster analysis by MEGA3.1.The genotypes of 48 materials showed high level of genetic diversity,with the sterile lines as parents,and good hybrid combinations could be obtained. |
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| Keywords: | sugarbeet SRAP molecule marker monogerm lines genetic diversity |
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