Comparative effects of ultrasonic transducers on medium chemical content in a nutrient mist plant bioreactor |
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Authors: | Yih-Juh Shiau Chien-Young Chu |
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Affiliation: | 1. Division of Biotechnology, Agricultural Research Institute, 189 Chung-Cheng Road, Wufeng, Taichung 413, Taiwan, ROC;2. Department of Horticulture, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan, ROC |
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Abstract: | The objectives of this study were to evaluate the efficiency of ultrasonic transducers in a nutrient mist bioreactor by analyzing the chemical constituents (carbohydrates and inorganic elements) of the mists produced by ultrasonic transducers from a liquid sucrose-supplemented Murashige and Skoog (MS) medium. The ultrasonic transducer oscillation frequency with an input power of 2.8 MHz and at 17.5 W was the optimum condition since the contents of glucose, sucrose, total soluble carbohydrate, macro-elements (NH4+-N, NO3−-N, P, K, Mg, Ca and S), micro-elements (Na, Fe, Mn, Zn, B, I, Mo and Co), and pH value were similar to those in the MS medium after autoclaving. Further, when MS medium was replaced by Gamborg et al. medium (B-5) or woody plant medium (WPM) in this bioreactor at the same transducer settings, the NH4+-N and NO3−-N contents in nutrient mist generated were also similar to those in B5 or WPM after autoclaving, respectively. The results of this research represent a significant contribution towards development of a functional nutrient mist bioreactor. |
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Keywords: | B-5, Gamborg et al. medium HMF, 5-hydroxymethylfurfural HPLC, high performance liquid chromatography ICP-AES, inductively coupled plasma atomic emission spectrometry ICP-MS, inductively coupled plasma mass spectrometry MHz, megahertz MS, Murashige and Skoog NMB, nutrient mist bioreactor OF, oscillation frequency PTFE, polytetrafluoroethylene PZT, plumbum zirconate titanate TSC, total soluble carbohydrate W, watt WPM, woody plant medium |
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