Effect of carbohydrates on in vitro low-temperature storage of shoot cultures of apricot |
| |
Authors: | Grazia Marino Paola NegriAntonio Cellini Andrea Masia |
| |
Institution: | Dipartimento di Colture Arboree, Università degli Studi di Bologna, Viale Fanin 46, 40127 Bologna, Italy |
| |
Abstract: | Procedures for cold storage of in vitro cultures can delay subculturing, reducing production costs and risks of contamination and somaclonal variation. The present work investigates the effects of media with sorbitol (116.8 mM, medium SO) or sucrose (58.4 mM) alone (medium SU), or the latter in combination with mannitol (58.4 mM, medium M) on 7-month storage at 5 °C of apricot shoots, cv San Castrese and Boreale. Shoots in SO survived in lower percentages and grew less than in the other treatments during storage, and died in large numbers after transfer to standard culture conditions. In comparison to other treatments, survival was 100% in the presence of M and both shoot weight and number of surviving proliferated axillary shoots was increased. Moreover, M improved regrowth compared to SU under standard culture conditions. The SOD and CAT activity confirmed the higher stress of shoots stored in SO than controls, and in contrast, the low stress of shoots in M. |
| |
Keywords: | Mannitol Punus armeniaca Shoot conservation Sorbitol Sucrose |
本文献已被 ScienceDirect 等数据库收录! |
|