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Investigation of biological behavior and colonization ability of Iranian indigenous phosphate solubilizing bacteria
Authors:Mandana Behbahani
Affiliation:Department of Biotechnology, Faculty of Advanced Science and Technologies, University of Isfahan, Isfahan 81746-73441, Islamic Republic of Iran
Abstract:Soil phosphorous (P) uptake mechanisms by the interaction of plants and soil microorganisms benefit plant growth with preparing agriculture inoculants. But it is influenced by soil factors. The present study was performed to identify three effective strains of phosphate solubilizing bacteria (PSB) on the basis of 16S rDNA sequence in vitro. Bacillus lentus strain PS5, Bacillus licheniformis strain PS7 and Pseudomonas putida strain PS13 were isolated from an alkaline soil in Iran and the rhizosphere of Beta vulgaris and Solanum tuberosom. Their high root colonization ability and P solubilizing activity was detected in relation to soil physical and chemical properties influenced by environmental factors including salinity of 800 mM/L and temperature of 42 °C.
Keywords:Phosphate solubilization   Bacillus lentus   Bacillus licheniformis   Pseudomonas putida   luxAB genes   16S rDNA sequencing
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