| ‘翠秀’黄瓜子叶原生质体的高效培养及植株再生 |
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| 引用本文： | 郭德章,鄢铮,赖钟雄,林庆良,王家福. ‘翠秀’黄瓜子叶原生质体的高效培养及植株再生[J]. 园艺学报, 2003, 30(2): 227-228 |
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| 作者姓名： | 郭德章 鄢铮 赖钟雄 林庆良 王家福 |
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| 作者单位： | ( 福州市农业科学研究所，福州350019; 福建农林大学亚热带果树研究所，福州350002) |
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| 摘 要： | 分离纯化的黄瓜子叶原生质体，以5.5×10～5.5×105个/mL的不同密度培养于mKMSp液体培养基中，均获得不同程度的细胞分裂。当培养密度超过1.0×103个/mL时，原生质体可持续分裂至愈伤组织形成，最高植板率为54%。原生质体来源的愈伤组织经诱导培养产生大量体胚并再生成植株。Ca2+ 浓度对黄瓜原生质体的稳定和细胞分裂有重要影响。
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| 关 键 词： | 黄瓜 子叶 原生质体 植株再生 |
| 文章编号： | 0513-353X(2003)02-0227-02 |
| 收稿时间： | 2002-05-21 |
| 修稿时间： | 2002-05-21 |
Efficient Culture and Plant Regeneration from Cotyledon Protoplasts of''''Cui xiu''''Cucumber |
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| Guo Dezhang ,Yan Zhen ,Lai Zhongxiong ,Lin Qinliang ,and Wang Jiafu. Efficient Culture and Plant Regeneration from Cotyledon Protoplasts of''''Cui xiu''''Cucumber[J]. Acta Horticulturae Sinica, 2003, 30(2): 227-228 |
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| Authors: | Guo Dezhang Yan Zhen Lai Zhongxiong Lin Qinliang and Wang Jiafu |
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| Affiliation: | (Fuzhou Institute of Agriculture of Sciences Fujian Province，Fuzhou 350014，China; Institute of Subtropical Fruit Trees Fujian Agricultural and Forestry University，Fuzhou 350002， China) |
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| Abstract: | Isolated and purified cotyledon protoplasts of cucumber(Cucummis sativust L． ‘Cuixiu’)were cultured at diferent population density ranging from 5.5×10/mL to 5.5×105 /mL in mKM8p liquid medium respectively an d underwent cell division in varying degrees．Sustained cel divisions with eventual somatic colony production occurred only when the population density of protoplasts exceeded 1.0×103/mL． the highest plating efficiency was about 54% ．Lager quantifies of embryoids diferentiated from protoplast derived ealli and then converted into planflets after succedent induction culture．Results demonstrate that the concentration of Ca2+ has an important effect on the stability an d cel division of cucumber protoplasts． |
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| Keywords: | Cucumber Cotyledon Protoplasts Plant regeneration |
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