Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method |
| |
Authors: | Fu-Hui Wu Shu-Chen Shen Lan-Ying Lee Shu-Hong Lee Ming-Tsar Chan Choun-Sea Lin |
| |
Institution: | (1) Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan;(2) Scientific Instrument Center, Academia Sinica, Taipei, 115, Taiwan;(3) Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA |
| |
Abstract: | Background Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant
genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity,
and in vivo protein-protein interactions. This method requires cutting leaves into very thin slivers to collect mesophyll cell protoplasts,
a procedure that often causes cell damage, may yield only a few good protoplasts, and is time consuming. In addition, this
protoplast isolation method normally requires a large number of leaves derived from plants grown specifically under low-light
conditions, which may be a concern when material availability is limited such as with mutant plants, or in large scale experiments. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|