小麦远缘杂交后代节燕98-2分离类型贮藏蛋白的研究

采用A-PAGE和SDS-PAGE聚丙烯酰胺凝胶电泳方法,对远缘组合分离出来的节燕98-2类型入选11个遗传性基本稳定的具有高产、多抗的小麦株系的醇溶蛋白和高分子量谷蛋白亚基进行了分析.结果表明,在A-PAGE电泳分析中,11个供试株系具有11种不同的醇溶蛋白带型.在SDS-FAGE电泳分析中,出现了7种不同的高分子量谷蛋白亚基(HMW-GS)及6种亚基组合类型,优质亚基及亚基组合所占的比例较少,品质评分偏低,其变幅为5～8分,平均为6.36分.但在所分析的材料中,出现了一个少见的特殊亚基:2 10 12.并研究了这些HMW-GS和组合频率及特点.11个株系中7个具有45 10优质亚基和2个具有2*亚基,它们可供小麦优质育种利用.研究表明,通过远缘杂交能够选育出具有高产、抗病和优质的小麦新材料.

Study of Storage Protein in One Separation Type JY98-2 Wheat of Spring of Distant Crossing

Using Acid-polyacry lamide gel electropherogram and SDS polyacry lamide gel electropherogram methods, the gliadin and high molecular weight glutenin subunit (HMW-GS) of 11 Wheat plant lines with high yield and various resistance were analyzed. The results show that there were 11 gliading genotypes in the 11 plant lines and that There were 7 subunits and 6 genotypes of HMW-GS in 11 plant lines,which the quality scores of these plant lines were quite low, ranging from 5 to 8, with the mean of 6. 36, but one special subunits, 2 10 12, was also found in plant lines of analysis. The frequency and characteristics of all of these HMW-GS and combination types were probed. Moreover, 7 plant lines carried 5 10 subunit and 2 plant lines carried 2* subunit were screened for application in the quality breeding program. The studies show new wheat materials with high yield, resistance and quality can be breeded by the distant crossing.