| 用基因枪法将抗除草剂bar基因导入小麦的研究 |
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| 引用本文： | 张媛媛,江昌俊,蒋明权. 用基因枪法将抗除草剂bar基因导入小麦的研究[J]. 南京农业大学学报, 2005, 28(1): 11-15 |
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| 作者姓名： | 张媛媛 江昌俊 蒋明权 |
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| 作者单位： | 安徽农业大学农业部茶叶生物化学与生物技术重点开放实验室,安徽,合肥,230031;安徽医科大学生物学教研室,安徽,合肥,230031;安徽农业大学农业部茶叶生物化学与生物技术重点开放实验室,安徽,合肥,230031;安徽农业大学生命科学学院,安徽,合肥,230036 |
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| 基金项目： | 安徽省“十五”科技攻关项目 (01013008) |
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| 摘 要： | 用基因枪法将含有抗除草剂bar基因的植物表达载体导入优良品种小麦幼胚愈伤组织，并通过GUS瞬时表达对沉淀剂、轰击距离、金粉用量等轰击参数进行了优化。用除草剂对轰击后的材料进行分化生根筛选，共获得33株再生苗。通过PCR检测和涂叶检测，初步证明其中4株已将bar。基因整合到小麦基因组中，平均转化率为0．33％。
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| 关 键 词： | 小麦 bar基因 抗除草剂 基因枪 |
| 文章编号： | 1000-2030(2005)01-0011-05 |
| 修稿时间： | 2004-02-16 |
Studies on transferring herbicide resistance bar gene into wheat by particle bombardment |
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| ZHANG Yuan-yuan,JIANG Chang-jun,JIANG Ming-quan. Studies on transferring herbicide resistance bar gene into wheat by particle bombardment[J]. Journal of Nanjing Agricultural University, 2005, 28(1): 11-15 |
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| Authors: | ZHANG Yuan-yuan JIANG Chang-jun JIANG Ming-quan |
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| Affiliation: | ZHANG Yuan-yuan 1,3,JIANG Chang-jun 1*,JIANG Ming-quan 2 |
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| Abstract: | The plant expression vector carrying the herbicide resistance bar gene was introduced into wheat immature embryo calli using particle bombardment.Parameters of bombardment were optimized by GUS transient expression,such as precipitants,distance,and amount of gold powder.After selection on media supplemented with different concentration of PPT,33 resistance plants were regenerated.It was demonstrated that the bar gene had been intergraded into 4 plants genome and expressed in transgenic plants by PCR analysis and spaying herbicide on the leaf.The average transformation frequency is about 0.33%. |
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| Keywords: | wheat bar gene herbicide resistance particle bombardment |
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