| 应用逆转录聚合酶链反应直接检测草鱼出血病病鱼组织的研究 |
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| 引用本文： | 李军. 应用逆转录聚合酶链反应直接检测草鱼出血病病鱼组织的研究[J]. 水产学报, 1997, 21(2): 175-179 |
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| 作者姓名： | 李军 |
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| 作者单位： | 青岛海洋大学海洋生物系 |
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| 基金项目： | 国家“八五”攻关课题!857220902，国家重点实验室和International foundation for science（A／2200-1）部分资助 |
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| 摘 要： | 人工感染ＧＣＨＶ－８６１后，对处于潜伏期、发病期和恢复期等不同时期的草鱼内脏组织匀浆上清液进行逆转录聚合酶链反应扩增，除恢复期的１条草鱼外，其余样品均到特异扩增带，对照组都没有，预示着ＲＴ－ＰＣＲ技术对于草鱼出血病的早期诊断、防治及抗病育种具有重要意义。
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| 关 键 词： | 草鱼 出血病 聚合酶链式反应 检测 |
| 收稿时间： | 2014-05-10 |
| 修稿时间： | 2014-05-10 |
Direct detection of grass carp hemorrhagic virus in infected fish by reverse transcription(RT)-polymerase chain reaction(PCR) |
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| LI Jun. Direct detection of grass carp hemorrhagic virus in infected fish by reverse transcription(RT)-polymerase chain reaction(PCR)[J]. Journal of Fisheries of China, 1997, 21(2): 175-179 |
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| Authors: | LI Jun |
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| Affiliation: | Department of Marine Biology, Ocean University of Qingdao, 266003 |
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| Abstract: | After artificial infected with GCHV-861, the grass carp were collected and detected during developing period, pre- and post-developing period by RT-PCR, respectively. All the infected fish gave positive results except one of five piece of covalenscent fish, and the controls are negtive.These indicated tha RT-PCR is significent for the early diagnosis and the viral disease-resistant breeding of grass carp- Moreover, we also first detected GCHV from the liver of infected hemorrhagic grass carp and discussed it. |
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