大蒜花梗组织培养再生植株

取生殖期大蒜花梗,在N_6+2,4-D 2mg/L+KT 1mg/L培养基上暗培养30天后,得到微黄色愈伤组织。将愈伤组织转到N_6+2,4-D 0.5mg/L+KT 0.5mg/L培养基上继代二次,然后于N_6+BA 5mg/L+KT 1mg/L+IAA 0.01mg/L,MS(1/2大量元素)+BA 5mg/L+KT 1mg/L+IAA 0.01mg/L和MS+BA 5mg/L+KT 1mg+IAA 0.01mg/L培养基上光照培养。3周后愈伤组织上出现绿色丛生芽,将小芽转到MS(1/2大量元素)+NAA 0.01mg/L培养基上,一周后发生白色根并形成完整植株。2,4-D和KT对大蒜花梗愈伤组织发生是必要的,高浓度的细胞分裂素和低浓度的生长素有利于芽分化,高浓度NH_4~+有利于根分化,低浓度NH_4~+有利于芽分化。愈伤组织低温处理对芽的分化是必要的。另外,对花梗愈伤组织发生进行了细胞学观察。

Plant Regeneration via Tissue Culture of Garlic Pedicel

Calli appeared from pedicels of garlic cultured on N6 + 2,4-D 2mg/L +KTlmg/L for 30 days. These calli survived two subcultures on N6 + 2,4-D 0.5mg/L+KT 0.5mg/L.Organogenesis occurred on N6 + BA 5mg/L + KT Img/L + IAA 0.01mg/L MS (1/2Macroelements) + BA 5mg/L + KT lmg/L + IAA 0.01mg/L and MS+ BA 5mg/L + KT Img/L + IAA 0.01mg/L.Roots appeared one week after the shoot wax transferred onto the MS(-|- Macroelements) + NAA 0.01lmg/L medium2,4-D and KT were essential to the induction of calli from garlic pedicels. Higher concentrations of plant growth regulators, i.e. cytokinin/auxin, lower concentration of NHJ and cold treatment to calli were all essential to shoot formation. Cellular observation on the process of callus induction from garlic pedicel is also disscussed.