| RAPD分子标记技术在茶树亲子鉴定中的应用 |
| |
| 引用本文： | 罗军武,施兆鹏,李家贤,沈程文,黄意欢,龚志华. RAPD分子标记技术在茶树亲子鉴定中的应用[J]. 湖南农业大学学报(自然科学版), 2002, 28(6): 502-505 |
| |
| 作者姓名： | 罗军武 施兆鹏 李家贤 沈程文 黄意欢 龚志华 |
| |
| 作者单位： | 1. 湖南农业大学食品科学技术学院,湖南,长沙,410128
2. 广东省茶叶研究所,广东,英德,513000 |
| |
| 基金项目： | 湖南省自然科学基金资助项目 (0 0 JJY2 0 10 7) |
| |
| 摘 要： | 为给茶树种质资源的鉴定、品种鉴定、亲子鉴定提供科学方法，通过对3个人工杂交组合的亲本及F1代进行RAPD分析。结果显示：双亲的RAPD谱带能在其F1代中表现出来，表现率分别为94.90%，97.92%,98.64%，说明3个杂交单株是其亲本的真正后代，这也表明RAPD技术在亲子鉴定中具有很强的适用性。同时从3个杂交组合的RAPD分析结果可以看出，双亲的RAPD谱带在F1代出现分离现象，这一结果表明茶树是一个杂合体。
|
| 关 键 词： | RAPD分子标记技术 应用 茶树 亲子鉴定 |
| 文章编号： | 1007-1032(2002)06-0502-04 |
| 修稿时间： | 2002-06-23 |
Study on the Application of RAPD Technique to Parentage Identification of Tea Plant |
| |
| LUO Jun wu ,SHI Zhao peng ,LI Jia xian ,SHEN Cheng wen ,HUANG Yi huan ,GONG Zhi hua. Study on the Application of RAPD Technique to Parentage Identification of Tea Plant[J]. Journal of Hunan Agricultural University, 2002, 28(6): 502-505 |
| |
| Authors: | LUO Jun wu SHI Zhao peng LI Jia xian SHEN Cheng wen HUANG Yi huan GONG Zhi hua |
| |
| Affiliation: | LUO Jun wu 1,SHI Zhao peng 1,LI Jia xian 2,SHEN Cheng wen 1,HUANG Yi huan 1,GONG Zhi hua 1 |
| |
| Abstract: | An analysis of three groups of hybridization combinations by artificial insemination shows that the RAPD bands in parents are nearly found in their offspring,with the appearance rate of 94.90% ,97.92% and 98.64% respectively, which proves that those three tea plants from three hybridization combinations are real hybrid offspring of their parents, and RAPD technique can effectively be used in Parentage identification, cultivars identification and germplasms identification.In addition, it can be found in this study that there are some separate phenomena of RAPD bands among the parents and their offspring. As a result,it can be concluded that every tree plant is a hybrid. |
| |
| Keywords: | Camellia sinensis random amplified polymorphic DNA parentage identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
