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秘鲁番茄与栽培番茄回交一代的获得及其染
色体片段分析
引用本文:赵鹤,王孝宣,杜永臣,高建昌,国艳梅. 秘鲁番茄与栽培番茄回交一代的获得及其染
色体片段分析[J]. 中国蔬菜, 2013, 1(14): 53-58
作者姓名:赵鹤  王孝宣  杜永臣  高建昌  国艳梅
作者单位:(中国农业科学院蔬菜花卉研究所,北京 100081)
基金项目:国家自然科学基金项目,国家‘863’计划项目
摘    要:以栽培番茄1052 为母本,以秘鲁番茄LA3858 为父本进行杂交,通过幼胚挽救培养获得了1
株F1 植株,再以栽培番茄1052 为母本进行回交,通过幼胚挽救获得了6 株BC1 单株,利用129 个分布于
12 条染色体上的CAPS、SSR 和InDel 标记对BC1 单株进行染色体片段分析,结果表明6 株BC1 单株中12
条染色体含有的秘鲁番茄片段涵盖了秘鲁番茄LA3858 的整个基因组。

关 键 词:番茄  秘鲁番茄  胚挽救  染色体片段  
收稿时间:2013-04-07;

Obtain and Chromosome Fragments Analysis of BC1 Populations between Ly-copersicon esculentum Mill. and L. peruvianum
ZHAO He , WANG Xiao-xuan , DU Yong-chen , GAO Jian-chang , GUO Yan-mei. Obtain and Chromosome Fragments Analysis of BC1 Populations between Ly-copersicon esculentum Mill. and L. peruvianum[J]. China Vegetables, 2013, 1(14): 53-58
Authors:ZHAO He    WANG Xiao-xuan    DU Yong-chen    GAO Jian-chang    GUO Yan-mei
Affiliation:(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
Abstract:AbstractIn this paper,cultivated tomato Lycopersicon esculentum Mill. 1052 was used as female
parent and wild tomato L. peruvianum LA3858 was used as male parent.One F1 hybrid was obtained
by embryo rescuing method.The hybrid was backcrossed with Lycopersicon esculentum Mill. 1052 to
generate BC1 progeny.And 6 single plants of backcross F1 species hybrids were obtained. Moreover,129
primers of CAPS,SSR and InDel on 12 chromosomes were used to analyze chromosome fragments of BC1.
The results showed that these 6 BC 1 plants contained the whole genome of L. peruvianum LA3858.
Keywords:Tomato  L. peruvianum  Embryo rescue  Chromosome fragments
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