小麦花药愈伤组织耐盐变异体的筛选

在培养基中加入一定浓度NaCl,对小麦F_1花药愈伤组织的耐盐变异体,进行细胞水平上的筛选.采用直接筛选法及逐渐增加盐浓度筛选法,结果表明,后者是提高耐盐变异体频率的有效措施.C_(17)+0.1%NaCl(转移愈伤组织)C_(17)+0.3%NaCl及C_(17)+0.1%NaCl(转移愈伤组织)C_(17)+0.5%NaCl处理的绿苗分化率,较直接筛选法分别提高5.06及15.0倍.在较高(加0.3%NaCl)盐含量的培养基上分化的花粉植株及其后代(H_2、H_3)表现耐盐性较强.获得了在0.4%含盐量土壤上种植能正常或基本正常生长的高耐盐株系3个.

Selection of Salt Tolerant Variants from Wheat Anther Callus Culture

By use of anther culture technique, salt-tolcrantc variants of winter wheat were screened at the cell level by adding quantitative NaCl into the medium C17 . About 143331 anther of F1 were cultured on the medium by di rect screening and inc-rcasing salinity screening method. The results proved that the later was a more effective measure for obtaining salt-tolerante variants. Green differentiation frequencies on C17 +0.1%, NaCl -(callus)-C17+0.3% NaCl and C17 + 0 . 1 % NaCl-callus-C17+ 0. 5% NaCl increased 5.06 and 15.0 times respectively, compared with direct screening method, by calculating the number of anthers cultured. Pollen plants differentiated on higher salinity medium C17 (added 0.3% and 0 . 5% NaCl) and their progeny ( H2 H1 ) have higher sal t-tolerance. They could develop normally or basically normally in the soil containing 0.4% salimity.