| 黄瓜耐低温基因转化后代的生物学鉴定 |
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| 引用本文： | 卢淑雯,刘文萍,李柱刚,南相日,刘建新,秦智伟. 黄瓜耐低温基因转化后代的生物学鉴定[J]. 中国蔬菜, 2010, 1(10): 16-19 |
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| 作者姓名： | 卢淑雯 刘文萍 李柱刚 南相日 刘建新 秦智伟 |
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| 作者单位： | 1.黑龙江省农业科学院，黑龙江哈尔滨 150086，;2.东北农业大学，黑龙江哈尔滨 150030 |
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| 基金项目： | 黑龙江省自然科学基金面上项目(C200611) |
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| 摘 要： | 对耐低温基因BnCS转化黄瓜植株T0种子及T1植株进行生物学鉴定,结果表明,经低温胁迫,黄瓜品系yd-36的转基因植株T0种子13℃的发芽势、发芽率及胚根长均显著高于非转基因种子;T1植株的电解质渗漏率和丙二醛含量也显著低于非转基因植株,株高、茎粗和叶面积的生长量均高于非转基因植株。证明转基因植株耐低温能力明显强于非转基因植株。
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| 关 键 词： | 黄瓜 耐低温基因 转化后代 生物学鉴定 |
| 收稿时间： | 2009-12-21; |
Biological Identification of Low Temperature Tolerance Gene Transform of Cucumber and Its Progeny |
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| LU Shu-wen,LIU Wen-ping,LI Zhu-gang,NAN Xiang-ri,LIU Jian-xin,QIN Zhi-wei. Biological Identification of Low Temperature Tolerance Gene Transform of Cucumber and Its Progeny[J]. China Vegetables, 2010, 1(10): 16-19 |
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| Authors: | LU Shu-wen LIU Wen-ping LI Zhu-gang NAN Xiang-ri LIU Jian-xin QIN Zhi-wei |
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| Affiliation: | 1.Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China;2.Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
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| Abstract: | Low temperature tolerance gene BnCS was transformed into cucumber（Cucumis sativus L.）plants. Biological characteristics of transgenic plant（T0）and its progeny（T1）were biologically identified. Under low temperature stress at 13 ℃, the germination potential, germination percentage and radicle length of transgenic T0 seed were significantly higher than the CK（cultivar yd-36）. The percentage of electrolyte leakage and MDA content（T1）were significantly lower than the CK. While the increment growth of plant height, stem width and leaf area were higher than the CK. This indicated that low temperature tolerance of transgenic plants was obviously higher than that of the non-transgenic plants. |
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| Keywords: | Cucumber Low temperature tolerance gene Transformed progeny Biological identification |
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