| Dynamic change of protein polypeptide of Ginkgo biloba seed during germination |
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| 作者姓名： | GUO Hong-yan LI Sheng-ping PENG Fang-ren |
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| 作者单位： | GUO Hong-yan，LI Sheng-ping，PENG Fang-ren* College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，P. R.. China |
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| 摘 要： | The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis （2-DE）. The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pIr.8, 31kD/pIr.8, 29kD/pIr.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins （VSPs）.
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| 关 键 词： | 银杏 种子萌发 发芽 蛋白质 多肽 动态变化 |
| 收稿时间： | 2005-10-06 |
| 修稿时间： | 2006-03-30 |
Dynamic change of protein polypeptide of <Emphasis Type="Italic">Ginkgo biloba</Emphasis> seed during germination |
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| GUO Hong-yan LI Sheng-ping PENG Fang-ren.Dynamic change of protein polypeptide of Ginkgo biloba seed during germination[J].Journal of Forestry Research,2007,18(1):35-38. |
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| Authors: | Guo Hong-yan Li Sheng-ping Peng Fang-ren |
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| Institution: | (1) College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, 210037, P.R. China |
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| Abstract: | The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis (2-DE). The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7kinds of proteins with different molecular weights and isoelectric points of 35kD/pI6.8, 31kD/pI6.8, 29kD/pI6.8, 33kD/pI6.6, 33kD/pI 6.4,34kD/pI7.7 and 31 kD/pI7.7 were identified primarily as vegetative storage proteins (VSPs). |
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| Keywords: | Ginkgo biloba seed Germination Protein Electrophoresis |
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