宽叶缬草根挥发油中乙酸龙脑酯的含量测定

[目的]建立宽叶缬草根挥发油中乙酸龙脑酯的气相色谱测定法。[方法]以萘为内标物,以浓度5%苯基甲基聚硅氧烷为固定相的弹性石英毛细管柱(30 m×0.25 mm,0.25μm)为色谱柱;检测器为FID检测器;检测器温度为250℃;气化室温度为200℃;柱温为150℃;载气为氮气;尾吹30 ml/min;进样量2μl。[结果]在色谱条件下,乙酸龙脑酯与内标物萘及其他挥发性成分的分离效果良好,乙酸龙脑酯浓度在0.393～3.146 mg/ml范围内与峰面积的线性关系良好(R=0.999 3),平均加样回收率为97.48%,RSD为1.1%(n=9)。[结论]该方法灵敏、准确、重现性好,可用于宽叶缬草根挥发油的质量控制。

Content Determination of Borneol Acetate in Volatile Oil from Roots of Valeriana officinalis by Gas Chromatography

[Objective] To establish the determination method of borneol acetate in volatile oil from roots of Valeriana officinalis by gas chromatography.[Method]Naphthalinum was used as the internal standard substance,the GC system consisted of capillary column（30 m × 0.25 mm,0.25 μm）,5% benz-methylpolysiloxane as the stationary phase,nitrogen as the carrier gas,and FID as the detector.The detector temperature is 250 ℃,vaporizer temperature 200 ℃,column temperature 150 ℃.[Result]Both borneol acetate in volatile oil and naphthalinum had got satisfactory separation under the chromatographic condition.The accuracy was at a perfect line range between 0.393 fmg / ml and 3.146 mg / ml,the mean recovery was 97.48%,and RSD was 1.1%（n = 9）.[Conclusion]The method is sensitive,accurate and reproducible,and it can be used to control the quality of volatile oil from roots of V.officinalis.