An inactivated vaccine for the control of bluetongue virus serotype 16 infection in sheep in Italy |
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Authors: | Savini G Ronchi G F Leone A Ciarelli A Migliaccio P Franchi P Mercante M T Pini A |
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Institution: | Department of Virology, OIE Reference Laboratory for Bluetonque, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise G. Caporale, Via Campo Boario, 64100 Teramo, Italy. g.savini@izs.it |
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Abstract: | Because no suitable products are at the moment available to safely control the spread of BTV-16 in Europe, an inactivated vaccine was produced from the reference field isolate of bluetongue virus serotype 16. One group of six sheep was vaccinated subcutaneously with the inactivated vaccine twice, on days 0 and 28, whereas a second group of eight sheep was inoculated with saline solution and used as mock-vaccinated control animals. Seventy-eight days after the first vaccination, all sheep were inoculated subcutaneously with a suspension containing 10(6.3) TCID(50) of a virulent reference BTV-16 isolate. Apart from a transient inflammatory reaction at the injection site, no adverse effects were reported following vaccination. All vaccinated animals developed high titres (7.3-9.3log(2)(ED50%/50 microl)) of virus-specific neutralising antibodies and were resistant to challenge with BTV-16. Conversely, following challenge, control animals developed hyperthermia and long lasting high-titre viraemia. |
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