| 铁皮石斛组织培养繁殖技术研究 |
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| 引用本文： | 郑慧俊,张明丽,邹春晶,龚仲幸.铁皮石斛组织培养繁殖技术研究[J].农业工程,2012,2(11):49-51. |
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| 作者姓名： | 郑慧俊 张明丽 邹春晶 龚仲幸 |
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| 作者单位： | 杭州职业技术学院，杭州310018 |
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| 基金项目： | 杭州市农业发展基金扶持项目(铁皮石斛的引种筛选和栽培技术研究) |
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| 摘 要： | 以铁皮石斛种荚为外植体，研究了不同消毒方式、培养基配方对于外植体生长的影响，探索最适于原球茎分化诱导、壮苗生长和生根的培养基配方。结果表明:有利于原球茎分化诱导的培养基配方是1/2 MS+2.0 mg/L BA+0.2 mg/L NAA；适合壮苗生长的培养基配方是MS+3.0 mg/L BA+1.0 mg/L NAA+1.0 mg/L KT+3%香蕉；适合幼苗生根的培养基配方是12MS+0.5 mgL NAA+3%香蕉+0.5%活性碳。
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| 关 键 词： | 铁皮石斛 组织培养 快速繁殖 培养基 |
Tissue Culture Technical of Dendrobium officinale Kimura et Migo |
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| Zheng Huijun,Zhang Mingli,Zou Chunjing and Gong Zhongxing.Tissue Culture Technical of Dendrobium officinale Kimura et Migo[J].Agricultural Engineering,2012,2(11):49-51. |
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| Authors: | Zheng Huijun Zhang Mingli Zou Chunjing and Gong Zhongxing |
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| Institution: | (Hangzhou Vocational & Technical College,Hangzhou 310018,China) |
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| Abstract: | Taking seed pods of Dendrobium officinale Kimura et Migo as exophyte，effects of disinfection ways and medium formula on exophyte growth were studied.Optimum medium proportions for protocorm induction，strong seedlings growth and rootage were explored.Results showed that 12 MS+2.0 mgL BA+0.2 mgL NAA solid culture medium was advantageous to induce protocorm，MS+3.0 mgL BA+1.0 mgL NAA+1.0 mgL KT+3% banana solid culture medium was suitable for strong seedling growth，and 1/2 MS+0.5 mg/L NAA+3% banana+0.5% active carbon solid culture medium was the best medium to induce root. |
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