抑制CCoAOMT表达的转基因多年生杨树检测与分析

本文以生长4年的转反义CCoAOMT基因的白杨杂种（欧洲山杨与银白杨，Populus tremula x Populus alba）及野生型对照为实验材料，测定Klason木质素含量并分析了木质素组分，结果表明转基因植株木质素含量经历4个生长季后，依然比对照降低13%，同时转基因植株木质素组分的G/S比比对照轻微降低。对茎杆切片显微观察表明转基因植株中部分导管向内凹陷，存在轻度变形。转基因植株与野生型对照在株高、胸径等外部生长形态上没有明显差别。

Analysis and Evaluation of Multiple-year-old Transgenic Poplar with Antisense CCoAOMT

Transgenic poplar (Populus tremula x Populus alba) with antisense CCoAOMT was planted in field for four years. The Klason lignin content evaluation showed that the transgenic tree had decreased lignin content by about 13% comparing to the wild control, which means that the decrement of lignin content was maintained in 4-year-old transgenic poplar. The lignin structure analyses by Thioacidolysis and GC displayed that G/S ratio in transgenic poplar was slightly decreased comparing to control. Histology examination of stem section showed that most of vessels in transgenic tree were regular similar to that in wild control, but some vessels transgenic tree were deformed with inward sunk walls. There were no difference in plant height and stem diameter between transgenic trees and wild control during four years of growing.