不同干扰生境中荒漠小灌木红砂种群遗传多样性研究

应用RAPD标记技术对荒漠小灌木红砂(Reaumuria soongorica)种群在不同扰动下的遗传多样性进行了分析。18条随机引物对红砂6个种群的120个个体进行扩增,共检测102个位点,其中多态位点99个。研究结果表明:红砂种群的多态位点比率(P)为96.86%,显示了不同生境中红砂种群内存在较高的遗传多样性。Shannon多样性指数(0.5007)、Nei基因多样性指数(0.3307)和基因分化系数(Gst=0.1952)揭示了红砂种群遗传变异多存在于种群内,种群间的遗传分化则较小。聚类分析表明:红砂种群遗传距离与地理距离之间存在一定相关性;遗传多样性水平与物种特性和不同干扰生境有关,与生态因子无相关性。

Analysis on the Population Genetic Diversity of Desert Shrub Reaumuria Soongorica in Disturbed Habitats

Genetic diversity of Reaumuria soongorica populations from the different disturbed habitats were assessed by means of random amplified polymorphic DNA(RAPD) markers.High genetic diversity and differentiation were revealed in the populations of R.soongorica by 18 random primers.120 individuals from 6 populations were sampled in the study.102 loci have been detected,and among them 99 were polymorphic.The mean proportion of polymorphic loci was 96.86%.The analysis of Shannon information index(0.5007),Nei's gene diversity(0.3307) and genetic differentiation among population(Gst=0.1952) indicated that there were more genetic variations within the populations than those among the populations.Cluster analysis showed that there was some correlation between genetic distance and geographic distance.The results showed that the genetic diversity was related to species traits and habitat disturbance,not correlated with ecological factors.