豫医无毛小鼠分离近交系的繁殖学特征及HLC小鼠近交系的建立

对豫医无毛小鼠和有毛小鼠生殖器官的大体形态、组织学结构及其繁殖能力进行了比较研究。结果表明，无毛小鼠生殖器官的位置、形态、组织学结构及发情周期与有毛小鼠无明显差异；无毛雄鼠明显具有生育能力，但其生育期短；无毛雌鼠有生育能力，但繁殖能力下降，受孕率较低，乳腺功能受损辑性差，不能很好地护理幼仔，幼鼠很难成活，一般1～3d内死亡。杂合子有毛小鼠的生育能力正常。在昆明小鼠繁殖群基础上，采用全同胞兄妹交配方式培育建立了HLC小鼠近交系，应用生化标记检测、皮肤移植试验和毛色基因测试法对育成的HLC小鼠进行遗传监测，证实其基因位点高度纯合；对其基本生物学特性进行研究，结果表明其为具有新特性的近交系小鼠，现已近交繁育30代。

Reproductive Peculiarities of Segregating Inbred Strain of Yuyi Hairless Mice and Breeding of a New HLC Inbred Strain Mice

The anatomo-histological structure and function of the reproductive system of Yuyi hairless mice were studied and contrasted to age-matched haired mice. The results showed that there were no significant differences between the hairless mice and haired mice in position,appearance,histological structure and estrous cycle. Hairless males are obviously fecund,but its reproductive stage is shorter. Hairless females are fertile as well,but reduced in reproductive capability,lower in pregnancy rates ,impaired in mammary gland function ,display abnormalities in maternal behavior ,and nurse its pups improperly. It is rare that the pups survive 3 days after birth. Haired heterozygous mice possess normal reproductive capability. The hairless mutant gene has pleiotropic effects,not only affects integument ,but also works on reproduction. Furthemore,through the full sib-mating inbred a new inbred strain mice named with HLC were established. Then,genetic monitoring was conducted by the skin grafting test ,cost color test and biochemical marker analysis, and its basic biological characteristics were studied with corresponding methods. The results indicated that the HLC mice with unique biological characteristics have been bred successfully and have progressed to 30th generation since 1991. The result of genetic determination showed that the gen.e loci should be homogenous. The HLC was proved to be an inbred strain fitting the international standards.