Bacterial community composition shifts in sediments influenced by fish feeds |
| |
| Authors: | Sheila Mae S Santander‐de Leon Sharon N Nuñal Hongyi Wei Takeshi Yoshikawa Hiroto Maeda |
| |
| Institution: | 1. Institute of Marine Fisheries and Oceanology, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, Iloilo, Philippines;2. Institute of Fish Processing and Technology, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, Iloilo, Philippines;3. Education and Research Center for Marine Resource and Environment, Faculty of Fisheries, Kagoshima University, Kagoshima, Japan |
| |
| Abstract: | This study investigated the influence of fish feed deposition on the microbial community of sediments in a coastal lake. Microcosms were designed to simulate the addition of fish feeds to the sediment, and physicochemical factors as well as composition of microbial assemblages were analysed. Although total organic carbon and total organic nitrogen did not significantly increase, redox potential and total sulphides showed a transition of sediment environments into anaerobic condition due to the addition of fish feeds. The bacterial community composition shifted with fish feed deposition as revealed by the band profiles of denaturing gradient gel electrophoresis, Sørensen's similarity indices and cell numbers of sulphate‐reducing bacteria. Occurrence of anaerobic bacteria (sulphate and nitrate reducers) and disappearance of strictly aerobic bacteria were observed with fish feed inputs. The results clarify the succession of the microbial flora and suggest negative impact of uneaten fish feeds on the microbial community in sediment and resultant environmental deterioration of fish‐farming areas. |
| |
| Keywords: | aquaculture bacterial community shift fish feed sedimentation PCR‐DGGE |
|
|
