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Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile
Authors:I Valdebenito  J Cosson  P Contreras  J C Sánchez  R P S Oliveira  J Risopatrón  J G Farías  E Figueroa
Affiliation:1. Núcleo de Investigación en Producción Alimentaria, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile;2. Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic;3. BIOREN – Center for Biotechnology in Reproduction, La Frontera University, Temuco, Chile;4. Estación Experimental Quillaipe, Fundación Chile, Puerto Montt, Chile;5. Biochemical and Pharmaceutical Technology Department, School of Pharmaceutical Sciences, University of S?o Paulo, S?o Paulo, Brazil;6. Departamento de Ingeniería Química, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, Chile
Abstract:Spermatological research of the Patagonian blennie was carried, specifically biometric parameters, sperm density, sperm count and motility in different activation mediums (815, 716, 590 and 0 mOSm kg?1), at different temperatures (5, 10 and 15°C) and pH levels (5, 7 and 9). The results indicate that Patagonian blennie spermatozoa have a primitive form, with a total length of 44.09 ± 3.36 μm, with a head length of 2.15 ± 0.28 μm and head width of 2.5 ± 0.31 μm. The mid‐piece had a length of 0.72 ± 0.12 μm, and its tail measures 41.21 ± 3.21 μm long. The motility pattern indicates that the spermatozoa are found immobile in the seminal plasma and only initiates its movement in a hypertonic medium from 590 to 815 mOsm kg?1. The longest motility time that was registered at 10°C in 716 mOSm kg?1 was of 245 ± 39 s and an optimum pH of 7 was observed.
Keywords:   Eleginops maclovinus     spermatology  sperm motility  osmolarity
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